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where k b is a bearing capacity factor that varies from 0.15 to 0.60 depending on the soil type and pile installation procedure (k b = 0.6 for piles driven into clay–silt and …

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S odium dodecyl sulfate polyacry-lamide gel electrophoresis (SDS-PAGE) is the most widely used ana-lytical method to resolve separate components of a protein mixture.

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1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1: 1-1 or 2+431-431-1=0+0+0+1 -- 1: 1'" 1 \ 1-1 or 3+431-431-1=0+0+0+1 -- 1: 1: 1-1 or 3 ...

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Gelatin (0.1%) or β-casein (0.1%) was mixed into liquid acrylamide when casting polyacrylamide gels. Concentrated conditioned medium, mixed with 2× Tris-glycine-SDS zymography sample buffer at 1:10 ratio, was loaded into 10% SDS-PAGE gels.

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XCell SureLock Mini Cell electrophoresis gel tank system ... When the OD 600 reaches 0.4–0.6, spin down cultures in a centrifuge rotor for 10 min at 11,900 ... Casting your own gel will not damage your sample, but care should be taken to avoid contaminating the gel.

Proteomic Identification of Novel Substrates of a Protein

The couplers for 0.6 in. diameter strands were tested to ultimate capacity so that the actual stress strain relationship of the coupler could be used in the moment curvature analysis co nducted on the prototype girder In addition, the results were compared with the ACI an d AASHTO LRFD requirements for post

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MAP-2 polyclonal antibody was then added (0.6 μg of sc-20172, Santa Cruz Biotechnology) for 1 h at 4 °C. Protein A-agarose (sc-2001, Santa Cruz Biotechnology) was added for 45 min at 4 °C to capture immune complexes.

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Characterisation of intact recombinant human

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You have free access to this content Characterisation of intact recombinant human erythropoietins applied in doping by means of planar gel electrophoretic techniques and matrix-assisted laser desorption/ionisation linear time-of-flight mass spectrometry†

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The mitochondrial respiratory chain is organized into dynamic high molecular weight complexes that associate to form supercomplexes. The function of these SCs is to minimize the production of ...

Wool fibril sponges with perspective biomedical applications

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Sheep's wool was used as a natural source to prepare keratin microfibril sponges for scaffolding, by disruption of the histological structure of the fibres through mild alkali treatment, followed by ultrasonication, casting and salt-leaching.

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OpenBSD 5.1 Changelog. ... Import of nginx 1.0.6 with a bundled libpcre. A possible replacement for Apache httpd(8) to be included in base. ... Make sure lock(9) lockmgr() use a different address for the wchan when draining the lock. Fixed a strlcpy(3) truncation test block in smtpd(8).

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Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting ...

Effects of hypochlorite exposure on flux through

Transfer a 1–5 μL aliquot of the glycoprotein stock solution to a 0.6-μL Eppendorf tube. 3. Incubate the glycoprotein solution at 95°C for 10 min to thermally denature the glycoprotein.

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Research highlights The water flux through polyethersulphone increased, but whey flux decreased, after exposure to sodium hypochlorite solutions. The rejection of whey protein decreased after exposure to sodium hypochlorite solutions. This showed that hypochlorite has an effect of PES membranes other than enlargement of pores.

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Gel-seq: A Method for Simultaneous Sequencing Library

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By casting several cassettes with several different layer volumes, researchers should be able to quickly determine the optimal configuration for their specific hardware. The Gel-seq protocol also has several critical steps that can be validated before the protocol is complete.

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